tnf-α, il-6  (Proteintech)

Journal: Materials Today Bio

Article Title: Engineered decellularized tendon hydrogel with sustained zinc ion release orchestrates anti-inflammatory microenvironment and functional regeneration in Achilles tendinopathy

doi: 10.1016/j.mtbio.2025.102104

Figure Lengend Snippet: Schematic illustration of Z-D hydrogel's dual functions in modulating the inflammatory microenvironment and facilitating tendon regeneration. The released ZnO NPs from Z-D hydrogel attenuate inflammation through inhibition of IL-6 and TNF-α expression, while promoting tendon repair by upregulating SCX, TNMD, and COL I expression alongside downregulation of COL III.(SCX:scleraxis; TNMD: tenomodulin; COL I:type I collagen; COL III: type III collagen; IL-6: interleukin-6; TNF-α: tumor necrosis factor-α).

Article Snippet: All stained sections were mounted with neutral resin and examined under a light microscope equipped with digital imaging capabilities, with particular attention to cellular infiltration, collagen alignment, and vascularization patterns.The immunohistochemical workflow involved sequential steps of slide deparaffinization, antigen retrieval via citrate buffer (Solarbio, China) microwave treatment, endogenous peroxidase blockade with 3 % H 2 O 2 (10 min), 37 °C trypsin digestion (2 h), and 10 % goat serum blocking, Followed this step, the sections were subjected to overnight incubation at 4 °C with primary antibodies targeting SCX (PA5-115874, Invitrogen), TNMD (ab203676, Abcam), COL I (A16891, Proteintech), COL III (A3795, Proteintech), IL-6 (21865-1-AP, Proteintech), TNF-α (21865-1-AP, Proteintech), CD86(13395-1-AP,Proteintech)and CD206(18704-1-AP,Proteintech).

Techniques: Inhibition, Expressing

Journal: Materials Today Bio

Article Title: Engineered decellularized tendon hydrogel with sustained zinc ion release orchestrates anti-inflammatory microenvironment and functional regeneration in Achilles tendinopathy

doi: 10.1016/j.mtbio.2025.102104

Figure Lengend Snippet: Inflammatory regulation of hydrogels. (A) IL-6 immunofluorescence staining in different groups under inflammatory induction; (B) TNF-α immunofluorescence staining in different groups under inflammatory induction; (C, D) Quantitative analysis of IL-6 and TNF-α immunofluorescence staining; (E, F) RT-qPCR results showing gene expression of IL-6 and TNF-α in the inflammatory microenvironment. (∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001).

Article Snippet: All stained sections were mounted with neutral resin and examined under a light microscope equipped with digital imaging capabilities, with particular attention to cellular infiltration, collagen alignment, and vascularization patterns.The immunohistochemical workflow involved sequential steps of slide deparaffinization, antigen retrieval via citrate buffer (Solarbio, China) microwave treatment, endogenous peroxidase blockade with 3 % H 2 O 2 (10 min), 37 °C trypsin digestion (2 h), and 10 % goat serum blocking, Followed this step, the sections were subjected to overnight incubation at 4 °C with primary antibodies targeting SCX (PA5-115874, Invitrogen), TNMD (ab203676, Abcam), COL I (A16891, Proteintech), COL III (A3795, Proteintech), IL-6 (21865-1-AP, Proteintech), TNF-α (21865-1-AP, Proteintech), CD86(13395-1-AP,Proteintech)and CD206(18704-1-AP,Proteintech).

Techniques: Immunofluorescence, Staining, Quantitative RT-PCR, Gene Expression

Journal: Materials Today Bio

Article Title: Engineered decellularized tendon hydrogel with sustained zinc ion release orchestrates anti-inflammatory microenvironment and functional regeneration in Achilles tendinopathy

doi: 10.1016/j.mtbio.2025.102104

Figure Lengend Snippet: Immunofluorescence Analysis.(A) IL-6 immunofluorescence staining in Achilles tendon tissues; (B) TNF-α immunofluorescence staining in Achilles tendon tissues; (C–D) Quantitative immunofluorescence analysis of IL-6 and TNF-α inflammatory expression intensities. (∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001).

Article Snippet: All stained sections were mounted with neutral resin and examined under a light microscope equipped with digital imaging capabilities, with particular attention to cellular infiltration, collagen alignment, and vascularization patterns.The immunohistochemical workflow involved sequential steps of slide deparaffinization, antigen retrieval via citrate buffer (Solarbio, China) microwave treatment, endogenous peroxidase blockade with 3 % H 2 O 2 (10 min), 37 °C trypsin digestion (2 h), and 10 % goat serum blocking, Followed this step, the sections were subjected to overnight incubation at 4 °C with primary antibodies targeting SCX (PA5-115874, Invitrogen), TNMD (ab203676, Abcam), COL I (A16891, Proteintech), COL III (A3795, Proteintech), IL-6 (21865-1-AP, Proteintech), TNF-α (21865-1-AP, Proteintech), CD86(13395-1-AP,Proteintech)and CD206(18704-1-AP,Proteintech).

Techniques: Immunofluorescence, Staining, Expressing